A project I worked on studying the transcription elongation factor Spn1 was published in Nucleic Acids Research. Spn1 is a transcription elongation factor which recent evidence suggests has histone chaperone activity. In this project, my collaborator constructed a system to deplete Spn1 from yeast cells, and used this system to study the in vivo functions of Spn1 by depleting Spn1 and assaying various aspects of transcription and chromatin state.
I analyzed the resulting genomic data, which included RNA-seq as well as ChIP-seq of RNA polymerase II (RNAPII), RNAPII post-translational modifications, transcription elongation factors, histones, and histone modifications. An archive of these data analyses is available at Zenodo.
-James